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| Synthesis and application of a fluorescent probe for the detection of reduced glutathione |
| Author Name | Affiliation | E-mail | | DU Yuting | Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi 034000, China | yutingdu123@163.com | | YUAN Jing | Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi 034000, China | | | DENG Peiyao | Department of Chemistry, Xinzhou Normal University, Xinzhou, Shanxi 034000, China | |
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| Abstract: In this study, a fluorescent probe, CDAS, was synthesized to detect reduced glutathione (GSH). CDAS was designed by incorporating triphenylamine as an electron-donating group and a 2,4-dinitrobenzenesulfonyl moiety as an electron-withdrawing group, with the response mechanism based on intramolecular charge transfer (ICT). These components were linked through Knoevenagel condensation. The probe exhibited no fluorescence; however, upon interaction with GSH, a nucleophilic substitution reaction occurred between GSH and probe CDAS, significantly enhancing fluorescent intensity at 561 nm. In a mixture of phosphate-buffered saline (PBS) and acetonitrile (1:1, V/V, 10 mmol·L-1, pH=7.4), CDAS demonstrated a low detection limit of 7.70 μmol·L-1, high selectivity for GSH, and a large Stokes shift of 179 nm. Notably, CDAS can be effectively utilized to detect GSH in human cervical cancer cells (HeLa cells) via fluorescent cell imaging. |
| Keywords: triphenylamine intramolecular charge transfer fluorescent probe reduced glutathione cell imaging |
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| DU Yuting,YUAN Jing,DENG Peiyao.Synthesis and application of a fluorescent probe for the detection of reduced glutathione[J].Chinese Journal of Inorganic Chemistry,2025,41(7):1331-1337. |
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