| 顺二氨二水合铂(Ⅱ)与膜磷脂及膜蛋白相互作用的荧光光谱研究 |
| FLUORESCENCE SPECTROSCOPIC STUDIES ON THE INTERACTIONS BETWEEN cis-DIAQUODIAMMINEPLATINUM(Ⅱ) AND THE MEMBRANE PROTEINS AND PHOSPHOLIPIDS |
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| 摘要: 用Tb3+分别对用和未用顺二氨二水合铂(Ⅱ)(AAP)处理的红细胞膜和磷酰胆碱(PC)脂质体进行荧光滴定,测Tb3+荧光。用AAP滴定红细胞膜,测蛋白质自身荧光变化。用间接Scatchard法求结合参数。表明蛋白质及磷脂均与AAP结合,蛋白质结合能力较强。 |
| 关键词: 顺二氨二水合铂(Ⅱ) 膜磷脂 膜蛋白 |
| 基金项目: 国家自然科学基金;国家教委博士点基金 |
| Abstract: The interactions between cis-diaquidiammineplatinum(Ⅱ), AAP and membrane proteins and phospholipids of erythrocytes were studied by titration of the crythrocyte membrane, intact and AAP treated, and phosphotidylcholine liposomc with Tb3+ by monitoring Tb3+ fluorescence. The membrane was also titrated with AAP by measuring the intrinsic fluorescence of protein. The binding parameters are obtained by Scatchard method. The results show that AAP binds to both proteins and phospholipids, but stronger with proteins. |
| Keywords: cis-diaquodiammineplatinum(Ⅱ) membrane phospholipids embrane protein |
| 投稿时间：1990-08-04 |
| 摘要点击次数: 1019 |
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| 王夔,刘冬.顺二氨二水合铂(Ⅱ)与膜磷脂及膜蛋白相互作用的荧光光谱研究[J].无机化学学报,1992,8(3):225-229. |
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