| 钴(Ⅱ)与金属硫蛋白的反应 |
| REACTION OF CO(Ⅱ)WITH METALLOTHIONEIN |
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| 摘要: 研究了Co(Ⅱ)和金属硫蛋白反应的动力学,发现反应分三段进行,由此得到了不同条件下各段对应的假一级反应速率常数.假设金属硫蛋白中每个金属结合20/7个SH基团,用盐酸滴定Co5Th(thioncin)和Co7Th,根据它们的紫外光谱特征波长处吸收值降至50%时的pH,分别140到它们的表观平均稳定常数.并且第一次发现当金属硫蛋白中钴逐步增加时.紫外吸收不断增强,但结合5个Co时即已达到应大,而不是7个Co.最后根据这些结果,对Co(Ⅱ)与金属硫蛋白反应的机理进行了讨论. |
| 关键词: 金属硫蛋白 动力学 钴 机理 表观平均稳定常数 紫外光谱 |
| 基金项目: 国家自然科学基金 |
| Abstract: The propertics of reaction of Co(Ⅱ)with rabbit liver MT have been investigated. The reaction is characterized by triphasic kinetics and the corresponding pseudo-first order rate constants arc determined under different conditions. Assuming each metal binding to 20/7 SH groups, average stability constants of Co7Th and Co5Th are obtained from hydrogen ions concentration required for 50% loss of the spectra absorbance. Interestingly,it was found that the absorption intensitics of Co-thiolate adduct, formed by adding Co to apoMT for varied ratio of co(Ⅱ)to MT, reach a maxium after 5 mol cquivalent co(Ⅱ)rather than 7 cobalt. All the above data give an deep insight into the mechanism of the reaction between cobalt and MT. |
| Keywords: metallothlonein kinetics cobalt mechanism apparent average stability constant UV-vis spectra |
| 投稿时间：1994-11-22 |
| 摘要点击次数: 948 |
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| 岳晟,仲维清,张保林,唐雯霞.钴(Ⅱ)与金属硫蛋白的反应[J].无机化学学报,1995,11(3):290-295. |
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