| 茜素红S-铜(Ⅱ)金属配合物与溶菌酶作用研究 |
| Study on Reaction of Alizarin Red S-Copper(Ⅱ) Metal Complexes with Lysozyme |
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| 摘要: 采用UV-Vis光谱法研究了pH=4.25的缓冲溶液中茜素红S(ARS)-铜(Ⅱ)金属配合物与溶菌酶(LYS)的结合反应。提出了双波长物质的量比法和平衡透析物质的量比法，与单波长物质的量比法进行对照测定研究，实验结果基本相符。研究发现，ARS-Cu(Ⅱ)-LYS的最大吸收波长为526 nm，比ARS红移96 nm，比ARS-Cu(Ⅱ)配合物红移10 nm。在526 nm处，测得ARS-Cu(Ⅱ)-LYS三元配合物的结合比为nARS∶nCu(Ⅱ)∶nLYS=6∶3∶1，摩尔吸光系数ε=7.93×104 L·mol-1·cm-1，ARS-Cu(Ⅱ)配合物与LYS作用的条件平衡常数K=3.21×1012。ARS-Cu(Ⅱ)与LYS之间的作用力为配位键和电荷力。 |
| 关键词: 双波长物质的量比法 溶菌酶 茜素红-铜(Ⅱ)配合物 茜素红S-铜(Ⅱ)-溶菌酶配合物 UV-Vis光谱法 |
| 基金项目: |
| Abstract: The interaction of Alizarin Red S(ARS)-Copper(Ⅱ) coordination compound with Lysozyme (LYS) was investigated by UV-Vis spectrophotometric method in acidic buffer solution(pH=4.25). ARS-Cu(Ⅱ)-LYS was a red color coordination compound having a maximum absorption at 526 nm with 10 nm, 96 nm and 96 nm of red shift compared to the ARS-Cu(Ⅱ) coordination compound, ARS complex and ARS-LYS complex. Dual wavelength substantial amount ratio method, Balance dialysis substantial amount ratio method and experimentation of unity wavelength substantial amount ratio method were suggested. The apparent substantial amount absorptivity of 7.93×104 L·mol-1·cm-1 was obtained. Combination proportion was defined as nARS∶nCu(Ⅱ)∶nLYS=6∶3∶1. Condition equilibrium constant K of ARS-Cu(Ⅱ) and LYS was 3.21×1012. It is suggested that combined dint between LYS and ARS-Cu(Ⅱ) were the coordinate bond and electrostatic force. Contemporary formation of electron transfer complexes is an importance factor in formation of ARS-Cu(Ⅱ)-LYS Comlexes. |
| Keywords: dual wavelength substantial amount ratio method lysozyme Alizarin Red S-Copper(Ⅱ) coordination compound Alizarin Red S-Copper(Ⅱ)-Lysozyme coordination compound UV-Vis spectra |
| 投稿时间：2004-07-26 修订日期：2004-10-09 |
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| 王兴明.茜素红S-铜(Ⅱ)金属配合物与溶菌酶作用研究[J].无机化学学报,2005,21(3):315-319. |
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